Immunostaining, Dehydration, and Clearing of Mouse Embryos for Ultramicroscopy
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چکیده
منابع مشابه
Immunostaining, dehydration, and clearing of mouse embryos for ultramicroscopy.
This protocol describes the preparation of mouse embryos for ultramicroscopy (UM), a powerful imaging technique that achieves precise and accurate three-dimensional (3D) reconstructions of intact macroscopic specimens with micrometer resolution. In UM, a specimen in the size range of ∼1-15 mm is illuminated perpendicular to the observation pathway by two thin counterpropagating sheets of laser ...
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ژورنال
عنوان ژورنال: Cold Spring Harbor Protocols
سال: 2013
ISSN: 1940-3402,1559-6095
DOI: 10.1101/pdb.prot076521